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KMID : 0380019940090040372
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 4 p.372 ~ p.376
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L-Lysine Production by 6-Azauracil Resistant Mutant of Corynebacterium glutamicum
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신현철/Shin, Hyun Chul
김성준/정영중/이재홍/Kim, Seong Jun/Jeon, Yeong Joong/Lee, Jae heung
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Abstract
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To improve L-lysine yield, pyrimidine base analogue( 6 -azauracil)- resistant mutants were isolated from Corynebacterium glutamicum KFCC10672. Among them, the best produce;C. glutamicum CH0516, was selected and tested for L-lysine production in a 7P fermentor. It was found that the product yield obtained with C. glutamicum CH0516 was higher than that of the parent strain by 3 %. In order to eluci. date the gain in productivity with the 6-azauracil-resistant mutant, enzymatic kinetic parameters such as aspartokinase(AKase) and aspartate carbamoyltransferase (ATCase) were measured. 7lic K,., v°1l ues of AKase with C. glutamicum KFCC10672 and CH0516 were 200.0 mM and 166.7 mM and thusc of ATCase were 0.13 mM and 0.27 mM, respectively. Howeve;the specific enzyme activities of Abase of C. glutamicum KFCC10672 and CH0516 were 3.89 x 10´ units/mg and 4.78 x 10´ units/mg, and those of ATCase were 2.20 units/mg and 1.84 units/mg, respectively. It appears that some increase in product yield with C. glutamicum CH0516 is likely due to the increased AKase activity and decreased ATCase acti% itc.
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